Cryo-EM Workflow

Single Particle Analysis Workflow

Biochemical Sample Preparation

Single-particle EM depends on the computational averaging of thousands of images of identical particles hence structural heterogeneity should be minimized through biochemical means to simplify structure determination. 

Although the single particle analysis workflow can alleviate partial heterogeneity in the specimen via 3D classification procedures, biochemical purification of the sample material to obtain a solution of isolated target proteins is required.

Cryo-EM specimens are typically prepared using several microliters of protein solution at a concentration ranging between 50 nM and 5 μM, depending on the specimen, EM grids and conditions used.

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