Analyze large, complex and flexible structures without crystallization.
Obtain structural information from mere nanocrystals of compound.
Acquire time-critical information for your increasingly challenging targets.
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Before the high-resolution data acquisition starts, the sample should be evaluated by cryo-EM (or perhaps better way of saying it: cryo-EM should be performed at a diagnostic level). The objective of this step is to qualitatively assess if the sample is promising to perform a 2D class average analysis and simultaneously obtain initial low-resolution map.
During this step the cryo-EM sample should be pre-screened to evaluate the following properties of the sample:
The cryo samples are ideally screened on the autoloader based cryo-TEM systems as multiple freezing conditions can be screened without compromising the ice quality. The Thermo Scientific™ Glacios™ is best suited for this purpose.
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